B.Tech Biotechnology Outline Syllabi
4 YEAR B.Tech. COURSES:
Thermodynamics and Chemical processes (10B11BT311)
Laws of thermodynamics, concept of entropy, Applications to compression and expansion processes. Solution thermodynamics - Excess properties of mixtures, Gibb's free energy, Gibbs-Duhem equation Chemical reaction equilibrium: Free energy and chemical reactions, equilibrium constant and its evaluation Phase equilibrium: Gibbs phase rule, fugacity as criterion of equilibrium, vapour-liquid equilibrium, completely miscible liquids. Thermodynamics of biomolecules, Chemical Processes - Importance of chemical process calculations, Material Balances in Systems Involving Physical Changes and Chemical Changes, Energy balances for non reacting and reacting systems.
Cell structure and function, Exploring biomolecules, Enzymes: Basic concepts and kinetics; Catalytic and regulatory strategies; Membrane structure and dynamics, Introduction to signal transduction cascades, Metabolic Energy: generation, storage and regulation strategies, metabolism & regulation of: carbohydrate, fatty acid, amino acid & nucleic acid, Metabolic disorders.
History of microbiology; Broad classification and taxonomy of microorganisms; Growth and physiology; Methods of microbial enumeration; Microbial control; Microbial metabolism, photosynthesis, fermentation, anaerobic respiration; Pathogenic microorganisms (bacteria, fungi, protozoa, and viruses, etc), host-pathogen interactions; Microbial genetics; Extremophiles; Microbes in industry.
Variation among organisms, Genes and Environment, Genotype and Phenotype, DNA-the hereditary material, Cell division, Mendelian genetics & beyond Mendelism, Chromosomes and Chromosome theory of inheritance, Linkage, crossing over and mapping, Extra Chromosomal Inheritance, sex determination and sex chromosomes, Mutations and their role in Evolution, Population Genetics, Genetic Disorders.
Cell Culture Techniques (10B11BT412)
Cell culture materials and tools, growth conditions and other requirements for establishment and maintenance of plant and animal cells, cell lines and tissues; in vitro conservation, protoplast & haploid culture, micropropagation; Animal cell cultivation: primary culture, growth kinetics, biology and characterization of cultured cells, Large scale production of biologicals in plant and animal cells.
Molecular Biology (10B11BT413)
Central dogma of molecular biology, nucleic acid structure: DNA and RNA, genetic code, DNA replication, Gene structure in prokaryotes and eukaryotes, Coding and Non coding DNA & RNA; molecular biology of transcription and translation; gene regulation, molecular mechanisms of recombination, transposons and rearrangement of DNA, DNA damage and repair, Post transcriptional and post-translational modification, Phage structure and biology.
Introduction to Bioinformatics (10B11BT511)
Scope of Bioinformatics and computational biology; Biological databases and various file formats; Sequence retrieval and submission. Homology search, sequence alignment and analysis using bio-algorithms such as dynamic programming. Gene and promoter predictions, challenges, strategies and tools evaluation. Phylogenetic analysis and tree evaluation. Sequence and structure analysis of RNA and Protein molecule. Applications of bioinformatics in drug discovery, pharmacogenomics, systems biology and next generation sequencing etc.
Process Engineering (10B11BT512)
Microbial process development, Bioreactor systems including utilities, Fluid flow and mixing - Flow behavior, mixing, power consumption and shear properties of rushton turbine, helical, anchor, bubble column, external loop, airlift etc. Heat transfer – different modes of heat transfer, Mass transfer in microbial processes, Enzyme Kinetics – Determination of rate parameters and reaction kinetics, Bioreactor analysis – Ideal and non-ideal reactor, Modes of culture – batch, fed batch, continuous, recycle. Fluidized bed bioreactors and immobilized bioreactors, Scale up of microbial processes with case studies related applications in various biotech and biopharma industries
Genetic Engineering (10B11BT513)
Concepts in Genetic Engineering, Enzymes in Genetic Engineering, Cloning Vehicles, BAC / YAC vectors, Construction & screening of genomic libraries, gene cloning strategies, DNA sequencing & mutagenesis, Cloning & expression of transgenes in Prokaryotic & Eukaryotic systems, PCR technologies, gene transfer in plant and animals, molecular markers, Applications and impact of rDNA technology, Ethical issues and biosafety regulation
Basic immunology, Types of immunity, B-cells and T-cells, Antibody: structure, function and diversity, T-cell receptors, Cellular and molecular aspects of antigens, Antigen-antibody Reaction, Regulation of Immune response and immunological tolerance, Immune effector mechanisms: Complement system and Cytokines, Autoimmunity and Autoimmune disease, Hyper-Sensitivity, Tissue and organ transplantation, MHC and HLA, Hybridoma technology, Immunity against infectious diseases, Vaccines, Immunodeficiency diseases.
Comparative and Functional Genomics (10B11BT611)
Genes and Genomes, high throughput genome sequencing, model genomes, genome annotation, Phylogenomics, Haplotyping, SNP technologies, proteomics, protein evolution, pharmacogenomics, gene expression profiling, global gene cloning & expression platforms and technologies, microarrays (DNA, RNA, proteins), gene-knock out & silencing technologies, protein-protein interactions, MALDI-TOF MS, LC-MS MS, high throughput identification of biomarkers.
Food and Agricultural Biotechnology (10B11BT612)
Food chemistry, microbiology of fermented food products, food spoilage and food borne diseases, Food processing and preservation, Current status of Indian processed food industry, Use of enzymes in food industry, nutraceuticals and functional foods, Single Cell Proteins, Probiotics, Biotechnological approaches in production of therapeutics & industrial products in animals and plants, Production of value added products (biofuel, bioplastics, etc) from agricultural waste, traditional crop improvement vs biotechnological interventions.
Cell and Developmental Biology (10B11BT613)
Biology of cell organelles and cytoskeleton, Membrane transport, Programmed cell death and senescence, Principles of developmental biology, Morphogenesis and organogenesis in animals and plants: Cell aggregation and differentiation in Dictyostelium discoideum, Early patterning and axis specification in Drosophila, amphibia and chick, Organization of shoot and root apical meristem; shoot and root development; leaf development and phyllotaxy,in A. thaliana, Vertebrate limb & organ development.
Fermentation Technology and Downstream Processing (10B11BT614)
Introduction to fermentation processes, Isolation, preservation and improvement of industrially important microorganisms, Media optimization, Bioprocess Considerations for animal & plant cell cultures, Downstream Processing : Filtration, centrifugation, Separation of insoluble products - Cell disruption : Physical methods, Chemical methods, Separation of soluble products- liquid-liquid extraction: solvent recovery, two phase aqueous extraction, Chromatography, Process design of Industrial Bio-products : Anaerobic bioprocesses - Ethanol and lactic acid production, Aerobic bioprocesses - Citric acid and penicillin production.
5 YEAR DUAL DEGREE B. Tech.- M.Tech. COURSES:
Cell Signaling and Signal Transduction (10M11BT111)
The machinery of signal transduction, Signal transduction pathways and their elucidation, Receptors and their targets, Control of cell signaling, Insulin function and diabetes, Regulation by Growth factors, cytokines & hormones; Cell regulatory programs, Transformation of normal cells to tumor cells, targets for new cancer therapeutics Analysis and manipulation of intracellular signaling via case studies
Metabolic Engineering (10M11BT211)
Signaling and complexity in gene networks; Stoichiometry, Synthesis of primary and secondary metabolites, Flux Balance analysis, Metabolic control analysis, Genetic Regulatory Networks, operons, regulons, stimulons, Regulation of Metabolic Networks, Resources, databases and software tools in metabolic engineering, Case studies and research paper discussion.
Diseases and Healthcare (10M11BT112)
Epidemiology, prevention & treatment of diseases: Bacterial (TB, Leprosy, Helicobacter) Viral (AIDS, Hepatitis) Protozoal (Malaria, Giardiasis), Helminths (Ascaris, Tapeworm), Fungal (Aspergillosis, Candidiasis), Neurodegenerative (Parkinson, Alzheimer, Spinocerebellar ataxia, Huntington’s disease, Multiple sclerosis, Amyotrophic lateral sclerosis), Others (Metabolic diseases, Cardiovascular Diseases, Respiratory Disorders, Genetic Diseases, Auto immune Diseases, Cancer), General health care
IPR and Patent Law (10M11BT113)
Types of intellectual property, patent law fundamentals, International IP treaties, patent search databases, revocation of patent, litigation and infringement, licensing and IP Management, International harmonization of patent law (WIPO), commercialization of biotechnology patents, Plant Breeders Rights, UPOV convention & protection of new plant varieties, IP protection for traditional knowledge.
Research Methodology and Data validation (10M11BT114)
Why study Research? Planning the study- Developing the study idea, Reviewing the Literature- Sources of Information, How to read a research article, Designing the Experiment, Steps in Conducting Scientific Research, Basic Statistical Concepts,- Descriptive Statistics, Inferential Statistics, Research, the law and you, Reporting your research.
Environment pollution vis-a-vis bioremediation, Nature and fate of environmental pollutants, Methods of bio-remediation (ex situ & in situ), Strategies in bioremediation, Biochemical pathways of degradation & monitoring of bioremediation, use of microorganisms in oil spill degradation and oil recovery, Remediation of gaseous effluents, Aerobic and anaerobic methods for sewage treatment, Metal bioremediation, Phytoremediation, GMO’s for bio-remediation.
Concepts in chemoinformatics and applications, Chemical structure representations and File formats. Graph theory and chemical structure searching algorithms, Chemical information system and databases viz, pubchem, chemdb, Drugbank etc., Bioinformatics tools for 3-D visualization and computation, Molecular similarity and diversity, Molecular descriptors, Chemical libraries and virtual screening, QSAR and computer aided drug discovery. High throughput chemistry, Pharmecophore modeling and biological activity.Comprehensive neural network based models in chemo informatics.
Diagnostic methods: X-rays, lumbar puncture, ultrasound, biopsies, arthroscopy, Hemoglobinopathies, Plasmapheresis, and Blood Banking; Diagnosis of Endocrine disorders; Diagnostic microbiology: Detection and characterization of bacterial and viral pathogens using molecular and immuno-diagnostic methods; Diagnosis of inherited Disorders; CNS diagnostics; Quality control: Laboratory standardization, quality control standards and monitoring.